CLARITY SOFTWARE - DATA APEX - Used Laboratory Equipment and New Lab Instrument Supplies, UK

CLARITY SOFTWARE - DATA APEX

Support of Multi-Detector measurement

Clarity is an advanced chromatography station designed to acquire and evaluate data from up to four chromatographs at a time. Clarity - a top product in its category - represents a universal solution for laboratories. It enables the user to acquire data from any commercially available chromatograph with standard analogue output.
Up to four chromatography systems, each of which can be equipped with up to four detectors, can be connected simultaneously. The chromatography station package includes an A/D converter (internal PCI card or external USB box) and the corresponding Clarity Software.

Clarity Station - simplicity and effectiveness

Clarity has been developed with a special emphasis on simplification and improved intuition of use. All frequently performed tasks are easily accessible thanks to the clear structure and intuitive graphical user interface. Users will be able to work immediately, without detailed training.

Acquisition and evaluation of data

Method and calibration files are the basis for acquisition and evaluation of chromatography data. Clarity performs calculations of all common non-calibrated and calibrated results. Instant response to a change of integration parameters or change in the calculation of results is definitely a great advantage.

Results

The Clarity chromatography station offers clearly presented results of obtained measurements. Displayed chromatograms, result tables as well as any subsequent printouts can be configured by the user.

Automation, Sequence

Connecting the Clarity station to chromatographs with auto samplers enables you to process data quickly and effectively, including automatic printing of results. Results are printed automatically and cross-samples are clearly presented in the summary table. Unattended sequence operation saves you time.

Operating systems

The Clarity System has been developed and tested for use with 32-bit operating systems Microsoft Windows 98, ME, NT, 2000 and XP.

Multitasking whilst acquiring data, you may work without problems on the evaluation and processing of already measured analyses or the preparation of parameters for subsequent analyses. It is naturally possible to run other programs whilst working with the Clarity station.

Support for GLP/ 21 CFR Part 11

In recent years, chromatography practice has brought about not only the necessity of efficiency in laboratory work, but also new demands relating to monitoring and security mechanisms, particularly in the field of data management. The Clarity supporting tools help you to comply with the requirements of GLP (Good Laboratory Practice) and FDA - 21 CFR Part 11.

Data Security A system of access rights and passwords is set up within the station. This system allows to create a unique password protected profile for each user. The user profile then defines in detail the users rights within the station (e.g. authority to effect changes in the methods of measurement) and may limit ones access to only certain connected instruments. Electronic signatures are also incorporated into the system so that a user may sign his or her data. This electronic signature is stored with the name and date and supplemented with a set phrase (e.g. measured by, approved by, etc.).

Audit Trail Detailed logs and histories of modifications enable users to maintain an audit trail. Clarity documents all parameters describing the conditions and methods of data processing for the user. This allows for easy access to a complete profile of information regarding any prior modifications performance.

Qualification Tools

The Test IQ (Installation Qualification) is an integral component of the station. This test monitors that the software has been properly installed and the results can be accessed from a printed protocol. Clarity Validator for OQ (Operational Qualification) is an optional package available for testing and validating the Clarity chromatography station. This is accomplished simply with the use of our chromatogram generator and a software utility.

Working with files

A Quick View feature within an Open Chromatogram window displays the curve and the chromatogram header. This helps the user easily navigate through previously saved chromatograms. Recently opened chromatograms are easily re-accessed through a configurable recent files list. Use Projects to structure your data and share it among other instruments. Clarity stations can share data through the computer network.

Layout

The core windows of the station use splitters (dividing content within one window e.g. graphs and tables) and tabs so that all information is conveniently laid out and easily accessed.

Configurations

Individual instruments are clearly, graphically configured in the Clarity station. Each user may customize his or her desktop settings (colours, fonts, graph lines, size and location of windows etc.).

Tables

Tables throughout the entire station share the same basic behaviour. However, users can adapt them to meet their requirements (width, visibility, column order and arrangement etc.). All can be adjusted to meet specific needs. Users can easily select available values of individual parameters from combo boxes. Frequently used functions such as highlighted copying of tables, are immediately accessible in a local menu. Data can be copied, not only among tables within the station, but also to other programs.

Automatic Update

Clarity enables you to use and display most files and results simultaneously at a number of places on the station. All information is automatically updated everywhere it is used with each alteration. This mechanism guarantees ultimate consistency and thus saves you the trouble of checking how current the data is.

Algorithm for detection of peaks

Highly responsive algorithms enable users to detect hundreds of peaks in each chromatogram. Data from the analysis can naturally be reprocessed without the necessity of repeating the analysis.

Integration

All integration parameters are saved in the integration table. Parameters such as Peak Width and Threshold are programmable in time. There are a total of 27 integration parameters incorporated to ensure the optimal integration of peaks. Most of these parameters can be applied also with the mouse from icons on the toolbar. Every change of basic integration parameters in the chromatogram window will force immediate reintegration.

Method

All parameters for data acquisition and peak integration, including baseline operations and link to the calibration file are saved in the template method. After creating of a chromatogram, a copy of the template method becomes a part of the chromatogram file.

Multi-detector measurement

Once a measurement has been completed within the framework of one instrument and more than one detector (up to four), signals are simultaneously displayed and accompanied by a legend identifying each signal and detector. Users may select the number of signals displayed according to preference.

Calibration

The Clarity Station uses linked calibration. Calibration curves are saved in a separate file, to which the template and chromatogram methods are referring. After updating or modifying this calibration file, results in all chromatograms linked to this file will be automatically recalculated upon opening. Although the calibration is linked, the current states of the calibration file and methods are stored in the chromatogram every time it is saved (using the commands Save, Print or Export). The chromatogram with its former method settings and calibration curves may thus be opened from any prior saved state.

Calibration calculations

Clarity offers the ability to calculate using external or internal standard methods. There are six types of curves to fit calibration points with the option to include a zero point. The station allows you to select automatic or manual (interactive) method of data transmission from the calibration standard into the calibration file. Groups of peaks may also be calibrated.

Method of reference peaks

Use reference peaks and or customisable identification windows for individual peaks to enhance the accuracy of compound identification.

Editing and recalibration

Browsing and adjustment of calibration data is simple thanks to the consolidation of all compounds in a single global calibration table. The calibration table and curve of each substance is placed separately on the relevant tab in the main calibration window.

Display of chromatograms

Users can easily zoom in to any section of the chromatogram. The then zoomed area can be further enlarged, reduced or moved in an auxiliary preview window. Return to the previous zoom using the commands Previous and Next Zoom. The appearance of the graph may be selected from an inexhaustible number of variants (colour, enlargement, annotation, axis selection etc.).

Custom labels

Text labels and lines can be attached to the chromatogram, and will automatically shift their position along with any movement of the chromatogram. The displayed chromatogram always contains a legend with the names of the file and detector in the upper right corner of the graph.

Display of multiple chromatograms

When working simultaneously with several chromatograms (Overlay mode) it is possible to perform basic mathematical operations including shifting, changing scale, calculating difference in sums and derivation. Three dimensional (3D) views for multiple chromatograms are also an option.

Results of analyses

The results of calibrated and non-calibrated analyses are shown as is common in analytical practice (height and area of peak, quantity of compound etc.). The Clarity station also offers calculations of parameters describing the quality of the chromatography system (asymmetry, efficiency, etc.). on the basis of two various methods of calculation. The extended possibilities of these calculations are provided by the Clarity SST module, which is an optional component of the station.

Special calculations

The Clarity station implements a powerful tool for creating and composing your own columns and choosing its calculation. If a station does not offer users a desired calculation, it can be easily created and defined. Values of all columns of a given table can be combined using 12 predefined mathematical operators, 15 basic and 4 summary functions and several global variables.

Summary table

The user-configurable summary table, displaying cross-sample data, presents a clear display of the results. The display of the summary table is highly configurable (display of individual parameters, orientation of lines and columns).

Print protocols

The Clarity station supports printing of user defined protocols. Its configuration is saved in report style, which defines the content and form of the printout. Pick from the predefined report styles or adjust them and store for further use. Different report styles can be predefined according to the dialog in which you are working. You can review the printout on the screen in the print preview. For printing protocols from multiple analyses users can either use batch processing of data or print a summary table.

Import and export

Clarity data is backward compatible with data of the CSW17 and CSW32 stations. It is also possible to import and export ASCII text and AIA data formats into/from Clarity. The results may also be exported to dBase format.

Sequence - Synchronisation with autosamplers

The Clarity station supports synchronisation with practically any type of auto sampler without the need to install a control module. Work is possible using both an active (activity controlled by the Clarity station) and passive sequence (activity controlled by auto sampler). Each sample in the sequence can be processed according to a different method. Certain samples can be marked as calibration standards so that they can be automatically used in the calibration or recalibration. This is especially helpful in larger sequences to maintain precision of the measurement. The ability to pause and modify a running sequence makes for great flexibility when working with sequence tables. The sequence file contains an audit trail, which records all events related to measurement. With direct control via the AS control module users can select his or her own method of injection, including selecting the order of vials.

Batch processing of chromatography data

The station offers tools for batch reprocessing of data (Batch), which users can employ in cases where it is necessary to identically process large amounts of analyses or to print out the results of several analyses simultaneously.

Post run functions

The Clarity station allows automated performance of routine operations following processing of data from each analysis. A processed chromatogram may be automatically displayed in the evaluation or calibration window, printed and results exported. It is also possible to define the activation of another program, which may further process the acquired data. Post run functions can be applied after batch processing of data, sequences or single analyses.

Archiving data

The station provides simple tools for backup and restore of chromatography data. Backup can be defined on the level of whole Projects involving a large number of analyses.

Key Parameters

Package: Software on CD, hardware A/D converter (internal PCI or external USB), hardware key, cables and manual

System configuration: Any 32-bit Windows system (98, Me, NT, 2000, XP)

Recommended configuration for Win 2000/ XP: PC Pentium/900 MHz, 256 MB RAM, monitor 1024x768, 64K colours (16 bit High Colour), CD ROM, free PCI slot/USB connector for A/D converter

Minimal configuration for Win 98: PC Pentium/200 MHz, 32 MB RAM, monitor 800x600, 256 colours, CD ROM, free PCI slot/USB connector for A/D converter

Number of instruments/channels: Up to 4 chromatographs (instruments) at a time, each up to 4 detectors (channels). 1 chromatograph (instrument) - 1 time base

Supported chromatographs: Any detector with voltage output, up to 30m distance

Measuring ranges: Bipolar: 156, 1 250, 10 000 mV, integration frequency up to 100 Hz, 24-bit resolution

GLP/ 21 CFR Part11 requirements: Password protection, Password expiration, Electronic signature, Audit trails, User account with access rights

Co-operation with auto samplers: The station cooperates with all autosamplers (as a passive measuring system or as an active measuring system), direct control available (refer to the list of controlled instruments)

Integration: Separate integration table for every detector, 27 integration parameters (peak width, threshold, tangent slope ratio etc.), integration parameters programmable in time, automated reintegration

Calculation types: Both without and with calibration (internal and external standard method), parameters of individual peaks for assessing efficiency of the column and the chromatographic system as a whole, SST Module for establishing deviations and reproducibility of some selected parameters

Calibrations: 6 types of calibration curves, up to 20 levels, reference peaks, groups, unlimited number of standards (peaks)

Work with chromatograms: Overlay, mathematical operations with chromatograms, custom labels and settings for chromatograms

Automation: Sequences, automatic launch of selected commands or applications immediately following chromatogram acquisition Post Run, Batch

Presentation of results: Integrated customisable table of results, columns with user-defined calculation, summary table, and export in text or database format

Custom calculations: 12 predefined mathematical operators, 15 basic and 4 summary functions

Protocol: Custom protocols, print preview option

Data export: ASCII, AIA, dBase

Data import: CSW17, CSW32, ASCII, AIA

Multi-user environment: Selectable system of user accounts with independently customisable behaviour and appearance for individual users

Network environment: Easy off-line (at the file level) data sharing among all stations in a local network

Add on modules, extensions: Control Modules, GPC, Test IQ, Validation Kit, SST Module, Clarity Eval (off-line version), LIMS Interface

Support, warranty: Automatic update from Internet, Free software updates, Free access to knowledge database, Online technical support, 3 years hardware warranty

Clarity hardware and software compatibility

In consideration of the many and variant operating systems, chromatographic stations and hardware available for data acquisition and in use today we offer the following summary table of possible Clarity software combinations:

INT7 - internal PCI Board of A/D Integration Converters

INT7 is an internal 24-bit A/D converter board that measures the voltages of chromatographic and electrochemical detectors. The board is designed for use with a PCI bus and equipped for easy Plug-and-Play installation. It contains 1 to 4 fully independent sigma delta integrating A//D converters for processing positive and negative voltages . INT7 is the direct successor to the classical ISA board INT5. The two share an identical design of analogue channels and thus, identical basic electric parameters.

The fundamental advantages include the following:

• Designed for all standard PCI slots
• Incorporated Plug-and-Play technology ensures easy installation with all the latest versions of Windows
• Four channel version directly on the board
• SMT technology makes the system more compact and reliable
• Improved temperature stability.
• In contrast to the INT5 converter, INT7 has re engineered the connectors wiring to provide a single input for each channel.
• INT5 - internal ISA Board of A/D Integration Converters

INT5 is an internal 24-bit A/D converter board for measurement of voltages of chromatographic and electrochemical detectors. The board is designed for the ISA bus and is equipped with mechanical jumpers for manual setting the base address and interrupt. It contains one to four fully independent, galvanically separated sigma delta integrating A/D converters for processing positive and negative voltages.

CB20 - internal PCI Board of D/A and D/f Converters

The CB20 board is a direct successor to the ISA board CB11. It contains four 16-bit D/A converters and four 28-bit frequency converters as well as eight auxiliary logical inputs and outputs. The board has been designed especially for control of analog-operated pumps by the chromatographic station CSW32 and Clarity. The board has no adjustable jumpers and, thanks to the employed Plug-and-Play technology, one has only to install the corresponding driver and set up the control parameters corresponding to the relevant pumps.

U-PAD - External USB A/D Converter

UPAD is a miniature external measuring unit designed for acquisition of data from any chromatograph for the CSW32 and Clarity station. UPAD uses the USB communication channel and thus is directly powered from the PC.

The unit can be connected or disconnected anytime without the necessity of shutting down the computer. The unit contains two independent channels with the latest 24-bit A/D converters capable of higher effective resolution especially at higher frequencies and at lower voltage ranges. The input connector is compatible with the internal INT7 board.

Net-PAD - External Ethernet A/D Converter

Net-PAD is an external high resolution A/D converter for chromatography data acquisition. Net-PAD communicates with a PC over Ethernet through TCP/IP protocol. Clarity Chromatography Station configured with Net-PAD converter enables a user to acquire chromatography data from distant instruments without the necessity to install additional cables. Net-PAD is compatible with any GC, HPLC, GPC, IC, CE system with standard analogue output. Clarity Chromatography Station can handle any combination of available Data Apex converters (internal PCI, external USB and TCP/IP).

SV8 - Terminal board

The connection between the Clarity Chromatography Stations A/D converters and the instruments is usually made using a cable that is supplied with the station. However, as the leads in the cable are soldered to an SUB-D 37 pin (CANNON) connector, any change in the wiring (for example adding additional leads to the digital OUT lines for controlling connected instrument functions such as gradient, fraction collector or the detector program start, detector auto zero or programmed valves switching) would requires some skill.

For users who will need to change the configuration of their instrument connections often, Data Apex offers the SV8 terminal board.

This boxed printed board has all the pins of the INT7 and U-PAD A/D converters conveniently available as screw contacts. For convenient access, the box is connected to the A/D Board with a 60 cm flat cable (supplied).

Note: Some interferences induced in the unshielded flat cable could not be excluded, however none were observed during thorough testing.

Validator

The Validator is a precise analogue signal generator device. The data is stored in the FLASH EEPROM memory in digital form and is converted to analogue data using a 1-bit D/A pulse width modulator. This converter guarantees full monotonicity, good linearity and no missing data points. The signal resolution is 20bit.

The Validator can be connected either directly to the DataApex A/D board 37-pin connector through the extension cable or through the terminal board to any other data acquisition device. Four sets of chromatogram data are stored in Validator.

Generated Data Perameters