Data Processing - Used Laboratory Equipment and New Lab Instrument Supplies, UK

Chromatogram - HPLC systems

Integration

This chromatogram shows the separation of a four component mixture that has been integrated using the integration parameters shown in the bottom window. The components are defined by retention time but not yet identified and named in the peak table, the peak starts and ends are also defined and tabulated. The integrated peak areas and peak heights are calculated and included in the peak table but the chromatogram has not yet been quantified by reference to a calibration curve. The dialogue box provides a means of quickly re-scaling the axes of the chromatogram if required.

Selecting a Component

Integration parameters are rapidly accessed and changed, when necessary, using a dialogue box that allows individual parameters in the integration process to be modified. By selecting a component in the peak table it is instantly identified in the graphical display, using a colour infill, so that peak definition by start and end points, using the current integration parameters can be viewed and quickly optimised. The four major components of the chromatogram are identified and named in the component table and peak number 3 (pyrene) has been quantified by reference to a prior calibration curve.

Integration

• Automatic Baseline
• Manual Baseline
• Drop Line
• Sloping Baseline
• Tangential Skimming
• Exponential Skimming
• Selectable Peak Detection
• Sensitivity
• Complete Operating Guide