Overlays - Used Laboratory Equipment and New Lab Instrument Supplies, UK

Normalised Overlays Confirm Purity

Automatic peak purity checks were made by the same upside and downside, on-the-fly, scans for all four peaks.

The results are shown for peaks 3 and 4 only, overlayed before and after normalisation of the spectral scans. Peak 4 is shown in the upper two plots and Peak 3 in the lower two.

From the overlays, particularly when normalised, it is seen that a near perfect match is obtained indicating that these peaks were homogenous and hence pure, as also were Peaks 1 and 2.

Eight Scans of Peaks

Several means of triggering the wavelength scan of a peak are user selectable.

In the example shown here, WaveQuest was programmed to make repetitive scans of pyrene as it eluted.

The use of the cursor is demonstrated by using it to measure the absorbance maximum at 326.7nm. The drop line shows that all the scans have the same wavelength for this absorption maxim.