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Spectral Matching

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Library Search Yields Identification

The use of the Spectral Library for peak identification is demonstrated by the chromatogram shown, which was obtained from a low cost lemonade style soft drink.

Comparisons were made between the UV spectra of the eluted peaks, with those of reference spectra which had been previously scanned and stored in the Spectral Library.

Overlay of these scans after normalisation indicated that these peaks were identical with Citric Acid (Peak 3), Saccharin (Peak 5), Aspartane (Peak 7) and Benzoic Acid (Peak 9).

Retention times for the major components coincided with those of the library spectra confirming the identifications.

The scan of Peak 9 is overlayed with the Library Spectrum of Benzoic Acid to show the very high quality of the spectral match obtained.

Benefits

  • Spectral Storage
  • Simple intuitive menu operation
  • Stackable with Adept HPLC modules
  • Flowcell replacement without adjustment
  • Validation software
  • Spectral Library
  • Library Search

Library Search Yields Identification

 
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