Perfect Vac Manifold-Single Basic - Used Laboratory Equipment and New Lab Instrument Supplies, UK

Vacuum source

Vacuum tubing

6 Protocol

Carefully unpack the unit and remove the set of adapters.

Familiarize yourself with the layout of the manifold.

Connect a vacuum line to the tube connector on the back of the unit.

Before each use of the manifold, be sure to inspect the unit and

make certain that the gasket and O-ring are in place, free of debris

and in good working condition.

1. Prepare samples as described in the Perfectprep Plasmid 96

Vac, Direct Bind protocol.

2. Remove manifold lid and place the short adapter plate in the

manifold chamber.

3. Place the FILTER PLATE DB over the adapter.

4. Place the manifold lid on the manifold base.

5. Position the FILTER PLATE A in the manifold lid. Make certain

that all plates are in the same orientation and FILTER PLATE A is

seated on the gasket.

6. When all of the FILTER PLATE A wells are loaded, turn on the

vacuum source.

amount of vacuum will vary with the nature of the samples, the

particular processing step in the procedure, and the vacuum

source.

8. After the vacuum has been applied for the time specified in the

protocol, release the vacuum by turning off the vacuum source.

9. Different combinations of plates and adapters will be used in

each subsequent step in the Perfectprep Plasmid 96 Vac, Direct

Bind protocol. Because the plates vary in height, the supplied

adapters should be used as specified in the Perfectprep Plasmid

96 Vac, Direct Bind protocol to prevent an empty space in the

vacuum manifold and maintain consistent vacuum pressure.

10. After completing the procedure, turn off the vacuum source and

clean off any spills with a damp paper towel. Do not use solvents

such as acetone, chloroform, etc. on any of the surfaces.

7 Routine Maintenance

The manifold will give the most reliable service when properly maintained.

After each use, any material spilled on the manifold lid (both

sides), the base, or in the chamber should be wiped off with a wet or

damp paper towel and then dried. Any debris on the gasket should

also be removed. If the O-ring starts sticking to the underside of the

lid, check that the O-ring and lid are clean. A very light coating of

vacuum grease (only) can be applied to the O-ring, should the problem

persist.

a. Make sure that the O-ring and gasket

are clean and free from debris.

b. Check the vacuum tubing and connections

to make sure there are no

leaks.

c. Visually inspect the plates to see if

there are cracks or nicks in the plastic.

d. Apply slight pressure to the plate and

the lid and hold for 20 to 30 seconds.

Some vacuum sources take longer to

generate sufficient vacuum.

e. Check that the samples have not

already been drawn through the

FILTER PLATE.

f. Make sure the gasket in the manifold

lid is positioned correctly. The gasket

should extend approximately 2 to

3 mm beyond the hole in the manifold

lid. The bottom edge of the plate

should sit on the gasket.