RHYS scientific — Science Technology Innovation — BTX ECM 630 Electroporation System - Used Laboratory Equipment and New Lab Instrument Supplies, UK

BTX ECM 630 Electroporation System

Description
This system incorporates features that make it the most technically advanced exponential decay electroporation system currently available. The generator utilizes the BTX Power Platform Technology design and novel digital user interface. The revolutionary Precision Pulse System provides the researcher unparalleled power in controlling the time constant. With the ability to deliver a maximum of 6000 A in the Low Voltage Mode, the ECM 630 is the most powerful generator in its class. Over 200 ECM 600 protocols may be duplicated with this instrument. The additional ECM 630 resistor selection "none" will allow researchers to reproduce protocols from competitive systems lacking resistor settings or reporting "unlimited" resistance. Indeed, the ECM 630 will perform the widest range of electroporation applications among commercial electroporators on the market.

Applications

Bacterial and Yeast Electroporation
The ECM 630 is the most flexible bacterial and yeast electroporator available, and will reproduce protocols by Beggs et al. for Mycobacterium avium ⁴; Sanders et al. for Haemophilus influenza and Haemophilus somnus ⁵; and Faber et al. for Hansenula polymorpha ⁶.

Mammalian Cell Transfections/ Protein/Drug Electroincorporation
Long duration electroporation, consistent with the ECM 630 platform, has resulted in higher expression levels in COS-7, EDR3, DG44, and CV1 cell lines ¹. The ECM 630 will duplicate the five-fold increase in transfection efficiency in comparison to calcium phosphate precipitation for COS-7 reported by Ishmael et al.² for the ECM 600, as well as the murine T Lymphoma results reported by Sundback et al.³.

Plant Applications
Intact plant tissue and protoplasts may be transformed using the ECM 630, including the species Agrostis palustris (creeping bentgrass) and Lycopersicon esculentum (tomato) ⁷, as well as tobacco pollen transformation⁸.

Other Cell Types
Exponential decay electroporation has been demonstrated to be superior to microinjection for medaka oocytes ⁹. Yee et al., transfected and expressed firefly luciferase in Giardia lamblia ¹⁰. In addition, Quillan et al. electrotransfected Xenopus laevis melanophores and fibroblasts ¹¹.

Specialty Electrode Applications: The BTX Choice
Takacs directed the electroporation of COS-7 in a 96-well plate format using single, 8-well and 96-well coaxial electrodes ¹², while Parham et al. optimized transient gene expression in CHO, CV-1, COS-7, HEK 293, and NSO using the BTX Flow Through Electroporation System ¹³. The coaxial electrodes have now been replaced by BTX High Throughput 96 Well Electroporation plate.

In Vivo Applications
Exponential decay generators may be used for a variety of animal in vivo applications, including drug delivery to pig carotid artery ¹⁴ and transdermal delivery of large molecules ¹⁵.

Technical Specifications

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